Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ, and J:ARC(S) Mice Following Exposure to Shedding Mice

Investigative plan. BALB/cJ (C) mice inoculated with and shedding Cm were cohoused with naïve C, C57BL/6J (B6), or J:ARC(S) [S] mice (1 infected C mouse with 4 naïve C, B6, or S mice per cage) for 30 d. Mice were monitored for 180 d. Three experimental and 1 control mouse per strain/stock were selected for qPCR, hemogram/serum biochemistry analysis, complete necropsy, and IHC at 3, 7, 14, 21, 63, 120, and 180 DPC. Additional mice were euthanized at 14 and 63 DPC for splenic (C, B6, and S) and gastrointestinal tract (B6 only) immunophenotyping.

Fecal shedding and intestinal colonization with Cm. (A) Fecal qPCR data (mean Cm copy number ± SEM) by time point and strain/stock (n = 3 to 6 mice/time point). *, Significant difference between C57BL/6J (B6) and BALB/cJ (C), and the J:ARC(S) [S] strain/stock (Kruskal-Wallis test, P ≤ .05). (B) Immunohistochemical staining for Cm MOMP. Cm antigen immunolabeling in surface epithelial cells throughout the colon (intracellular brown staining) of B6, C, and S mice at 7 and 180 DPC. Cm inclusions are noted in the cytoplasm of enterocytes (insets). Immunolabeling is detected in B6 and C mice at 7 DPC and all strains/stocks at 180 DPC. Staining of luminal contents reflects nonspecific staining of extracellular debris Immunohistochemistry for MOMP antigen, 2× (insets: 10×).

A subset of Cm-infected mice exhibited mild colitis at 180 DPC. (Top) Representative histology of the colon from uninfected (control) C57BL/6J (B6), BALB/cJ (C), and J:ARC(S) [S] mice demonstrating normal intestinal mucosa and submucosa. H&E, 60×. (Bottom) Subset of Cm-infected B6 and S mice developed mild multifocal colitis at 180 DPC. The lamina propria and/or submucosa was infiltrated by lymphocytes, plasma cells, (yellow arrowheads), histiocytes, and less frequently neutrophils. No colitis was observed in Cm-infected C mice. Inset: Cm MOMP immunolabeling (dashed box) in the cytoplasm of colonic enterocytes from infected C mice. H&E, 60× (inset: immunohistochemistry for Cm MOMP antigen).

Cm large intestinal colonization is associated with GALT. (A) GALT hyperplasia scores (mean ± SEM) at 14 and 63 DPC in C57BL/6J (B6; n = 3/time point), BALB/cJ (C; n = 6/time point), and J:ARC(S) [S; n = 6/time point] mice. No significant differences in GALT hyperplasia were appreciated due to a small sample size; however, infected mice had higher scores at both 14 and 63 DPC. Representative examples of normal (0), mild (1), moderate (2), and marked (3) GALT hyperplasia in the large intestine from S mice at 63 DPC are presented. *, Prominent germinal centers. H&E, 5×. (B) Association between Cm-infected epithelial cells and GALT in B6, C, and S mice (n = 3 to 6 mice/time point). Cm infection of epithelial cells overlying GALT provided in images on left. Arrowhead is an intraepithelial Cm inclusion; Cm MOMP antigen (brown staining) is present in epithelium overlying GALT in photomicrographs. IHC for MOMP. 2× (insets: 20×). GALT association scores (mean ± SE) in the cecum and colon of B6, C, and S mice at various times post-cohousing in the histogram on the right. *, Significant differences (P ≤ 0.05) between each strain/stock; ^, significant differences (P < 0.05) between B6 and C mice; ?, significant differences (P < 0.05) between B6 and S mice.

T-cell subsets in small and large intestinal GALT. Representative immunohistochemistry for CD4, CD8, and Foxp3 T cells in the GALT of C57BL/6J mice at 14 DPC. Infected mice had T-cell populations comprised of primarily CD4-positive cells (left: brown cytoplasmic/membranous immunolabeling of round cells) with less frequently observed CD8-positive cells (middle: brown cytoplasmic/membranous immunolabeling of round cells). Both CD4- and CD8-positive cells were often found clustering in interfollicular regions between germinal centers and scattered in the mantle zones, perifollicular regions, and associated lamina propria. The small intestine from the uninfected control mice had similar immunolabeling of CD4- and CD8-positive lymphocytes as found in infected mice. The lymphocyte populations of CD4 and CD8 cells in the large intestine were more homogenous in uninfected controls. Both infected and uninfected mice presented with infrequent T-regulatory cells (assessed via Foxp3; right: brown nuclear immunolabeling). Immunohistochemistry for CD4, CD8, and Foxp3, 5× (insets: 20×).

ISH staining for Cm in the gastrointestinal tract, lungs, and uterus. (A) Cm nucleic acid (punctate dot red staining, arrowheads) is detected in apical epithelial cells of the colon in a Cm-infected C57BL/6J mouse, 63 DPC. ISH for Cm, 10×. (B) Lungs from an Cm-infected J:ARC(S) [S] mouse at 63 DPC demonstrating no Cm nucleic acid hybridization. ISH for Cm, subgross. (C) Cm nucleic acid is not detected in the uterus from a Cm-infected S mouse at 14 DPC. ISH for Cm, subgross.

Immunophenotyping of splenic immune cells during early and late stages of Cm infection in C57BL/6J (B6) mice. (A) Gating strategy defining the immune subpopulations of splenic cells after excluding debris, doublets, and dead cells. Splenic immune cells were first identified as CD45⁺ cells. B cells were then quantified as CD3⁻/B220⁺. T cells were identified as CD3⁺. CD8 and CD4 T cells were identified by expression of their respective marker, while the subpopulation of T_reg was identified as CD4⁺/CD25⁺/CD127⁻. CD4⁺ and CD8⁺ T cell activation was identified as CD69⁺. Neutrophils and monocytes were quantified as CD11b+ and further distinguished as Ly6G+ and Ly6G–Ly6C+, respectively. Data displayed are from B6 mice at 14 DPC. (B) Infected B6 mice demonstrated increased frequencies of myeloid and lymphoid cell population subtypes when compared with uninfected controls, including monocytes (63 DPC), effector CD4+ T cells (14 and 63 DPC), effector CD8+ T cells (63 DPC) and effector memory CD8+ T cells (14 DPC). Other population increases did not reach statistical significance. In addition, infected B6 mice had fewer B cells (63 DPC), naïve CD4+ cells (14 DPC), and overall CD8+ T cells (14 FPC). Data represent the frequencies of specific cell populations as compared with the parent population and are displayed as the proportions of average values in infected as compared with control mice (n = 6 mice/group). Dotted line (1.0) indicates no difference in frequency of detection between control and infected mice. Values above this line represent an increased frequency of detection in infected mice and values below this line represent a decreased frequency of detection in infected mice. CM, central memory; EM, effector memory cell; macro, macrophage; mono, monocyte; neut, neutrophil; TIC, total immune cells. *, P ≤ .05.

Immunophenotyping of splenic immune cells during early and late stages of Cm infection in BALB/cJ (C) and J:ARC(S) [S] mice. (A and B) Data represent the frequencies of specific cell populations as compared with the parent population and are displayed as proportions of average values of infected as compared with control mice (n = 6 mice/group). Dotted line (1.0) indicates no difference in frequency of detection between control and infected mice. Values above this line represent an increased frequency of detection in infected mice and values below this line represent a decreased frequency of detection in infected mice. (A) Infected C mice demonstrated increased frequencies of myeloid and lymphoid cell population subtypes when compared with uninfected controls, including monocytes (63 DPC) and central memory CD8+ T cells (63 DPC). In addition, Cm-infected mice demonstrated fewer macrophages and CD4+ T cells when compared with uninfected controls at 14 DPC. (B) Infected S mice demonstrated increased frequencies of myeloid and lymphoid cell population subtypes when compared with uninfected controls, including monocytes (63 DPC), effector CD4+ T cells (63 DPC), effector memory CD8+ T cells (63 DPC), and naïve CD8+ T cells (14 DPC). In addition, infected mice demonstrated fewer naïve CD4+ T cells and effector CD8+ T cells when compared with uninfected controls at 14 DPC. CM, central memory; EM, effector memory cell; macro, macrophage; mono, monocyte; neut, neutrophil; TIC, total immune cells. *, P ≤ .05.

Immunophenotyping of large intestinal immune cells during early and late stages of Cm infection in C57BL/6J (B6) mice. (A) Gating strategy defining the immune subpopulations of the large intestinal cells after excluding debris, doublets, and dead cells. Immune cells were first identified as CD45⁺ cells. T cells were then identified as Lin1⁺ and ILCs as Lin1⁻. CD4 T cells were identified by expression of CD4 and further classified as effector (Tbet) or regulatory (Foxp3) CD4⁺ T cells. ILC3s were identified as CD90 and CD127⁺. Data displayed are from B6 mice at 14 DPC. (B) Representative flow cytometric analyses from CD4 T cell and ILC3 subsets. There were significantly more total immune cells (CD45⁺) in the large intestine of infected mice compared with uninfected controls at both 14 and 63 DPC. In addition, the cells isolated from the large intestine of infected B6 mice demonstrated increased frequencies of several CD4⁺ T cell and ILC subtypes associated with Th1 and Th17 cell immune responses when compared with uninfected controls. These mice had more frequent T_eff CD4⁺ cells (14 and 63 DPC) and fewer T_reg cells (14 DPC). These T_eff cells more frequently expressed both Tbet and RORγ (63 DPC). In addition, ILCs and the ILC3 subtype more frequently expressed T-bet (63 DPC), and the ILC3 subtype also more frequently expressed CCR6 (63 DPC). Finally, the large intestine immune cells more frequently expressed several cytokines (with ILC3 having more frequent GM-CSF at 63 DPC, IL-17A at 14 DPC, and IL-22 at both 14 and 63 DPC, and CD4⁺ T cells having more frequent IFNγ, IL17A, and IL22 at both 14 and 63 DPC). Data represent the frequencies of specific cell populations as compared with the parent population and are displayed here as proportions of average values of infected as compared with control mice (n = 6 mice/group). Dotted line (1.0) indicates no difference in frequency of detection between control and infected mice. Values above this line represent an increased frequency of detection in infected mice and values below this line represent a decreased frequency of detection in infected mice. CM, central memory; EM, effector memory cell; macro, macrophage; mono, monocyte; neut, neutrophil; TIC, total immune cells. *, P ≤ .05.

Cytokine production in Cm-infected C57BL/6J mice at 14 and 63 DPC. Cm-infected mice produce significantly more IL-6 than uninfected counterparts at 14 DPC, while at 63 DPC, Cm-infected mice produce greater levels of both IL-4 (associated with Th2 responses) and IL-12p70 (associated with Th1 responses) as compared with uninfected controls. IL-12p70 is markedly elevated. In addition, Cm-infected mice exhibited an increase in IL-4, IL-10, and IL-12p70 from 14 DPC to 63 DPC. Data represents a single experiment with 6 mice/group. Bars represent the SEM. *, P ≤ 0.05.